Though most of the work is over, the final morphometric analysis of the slides prepared from the mice testis is still pending.
For this assistance of the department of pathology is sought. They too are willing. I must accommodate some time at least next week for this.
The following parameters are intended to be measured in the slides prepared so far, using a morphometric microscope.
10 semeniferous tubules which are cut almost transversely in each slide is taken for the account. Three such observations will be done from each of the testis from non consecutively cut sections.
For this assistance of the department of pathology is sought. They too are willing. I must accommodate some time at least next week for this.
The following parameters are intended to be measured in the slides prepared so far, using a morphometric microscope.
10 semeniferous tubules which are cut almost transversely in each slide is taken for the account. Three such observations will be done from each of the testis from non consecutively cut sections.
- Semeniferous tubular diameter, surface area and perimeter
- Maximum epithelial cell height from the tubular basement membrane
- Sertoli cell nuclear diameter
- Myoid cell count
- Minimum and maximum interstitial space length
From the sperm smear slides the following are intended to be noted.
- Sperm head shape variations.
- Decapitated sperm heads per 1000 sperms counted
- Vacuolation of sperm head per 1000 sperms counted.
Mean while we had a MCI inspection in our college, to which my honorable guide has attended and he generously signed the declaration page to be pinned with my thesis copy later on.
