Every day I and BK sir use to visit the animal house at least once, for giving oflox to the rat. After one set of rats were sacrificed, the new ones are fractured, drug is started in a week’s time. We failed to visit the animal house last Monday.
And that day- 4 of the mice were dead, for the reasons unknown. We got the news on the following say. I gave my personal number to the ‘akka’ incharge of the animal house and asked her to inform the news of any animal death in there irrespective of cause of death. She understood that people from anatomy department are after dead – anything dead!
This Monday I got the call at 8 am, akka said, “ 2 mice are dead”. I was relieved that I don’t have to kill normal mice on my own to run my control study.
I was getting ready for the dissection – found that Ajay too interested in the mice dissection. BK sir joined. We dissected both the mice. We took out the testis, pancreas and liver. Sperm extraction from the epidydimis was not possible as it was already shrivelled.testis is put in Bouins solution and rest organs in the formalin. It’s still with us now. Tomorrow will hand it over to pathology for the histological study.
This will be my first mice tissue sample collection.
Today I am going to get the drug Gemvita, from Gopal Kamath wholesale distributors. It will cost me Rs. 1150 for 200 mg amp. It can be stored at room temperature. But the problem is it said it has to be used within 24 hrs of reconstitution.
In our animal house there is no proper ventilation and temperature maintenance. This could be the probable reason for the death of the mice, as no one attends them on whole of Sunday. They are not given water and food, and not even fresh air on Sundays. A strict holiday. In 2 consecutive weekends we have lost 6 mice.
No comments:
Post a Comment