Some how the department of Pharmacology is persuaded and they agreed to give the aged mice - which they are not using. Yesterday Prof and Head of Pharmacology directed Akka to give the mice. I received 10 male and 9 female Swiss albino mice in two cages. I made the necessary purchasing of diluent for Inj. Gemita - that's NS, with 20 insulin syringes.
Today - with BK sir and Santhosh sir we injected the mice with Gemcitabine and divided them into 2 groups - those which got 4mg and those which got 8mg!
No. of mice injected with drug | Date | Approximate amount | Number of mice sacrificed to be on | ||
30-11-10 | 07-11-10 | 30-01-11 | |||
6 male mice | 23-11-10 | 4 mg each | 2 | 2 | 2 |
4 male mice | 23-11-10 | 8 mg each | 2 | 2 | |
5 female mice | 23-11-10 | 4 mg each | 2 | 2 | 1 |
4 female mice | 23-11-10 | 8 mg each | 2 | 2 | |
Totally 19 mice are subjected to the study and we have diluted the entire drug in the ampule !!
We are left with more than half of the drug - most probably will be discarded tomorrow !
Tomorrow will be posting the photos !
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