Write up of thesis

Though the thesis is not yet complete, I have started the write-up work. Only probable remaining work in the thesis is sacrificing, dissection and histology of so called group D mice, wherein it is expected to see a reversal of drug effects.
It may be followed by sacrifice of offsprings of mice treated with drug; if there are any changes, its a delight.

these are the changes seen in histology.

testis of control mice.

testis of mice treated with 4 mg of gemcitabine.

changes identified so far
1. decrease in seminiferous tubule morphology - decrease in its diameter. quantification required and significance has to be established.
2. change in the cytoarchitecture of seminiferous tubules
3. Interstitital tissue has decreased. But tightly packed with cells
4. large nucleoli seen in spermatogonial cells
5. Vacuoles seen in few spermatogia.
6. Probable increase in sertoli cell
7. Probable increase in Leydig cells.
8. Decrease in spermatids.
9. Uniform hyalinized appearance of the lumen of tubules.
10. thickening of basement membrane of tubules.
11. decrease in height of epithelial cells.

these have to quantified.

Sperm shape abnormalities:

apparently normal sperms

mice treated with gemcitabine

observations so far -

1. many of the heads are decapitated.

2. loss of banana tailing.

3. vacuolation of the cytoplasm in the head.

these things again has to quantified and significance has to be proved with reference to normal.